Enzymatic synthesis of structure-free DNA with pseudo-complementary properties
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چکیده
منابع مشابه
Enzymatic synthesis of structure-free DNA with pseudo-complementary properties
Long single-stranded DNAs and RNAs possess considerable secondary structure under conditions that support stable hybrid formation with oligonucleotides. Consequently, different oligomeric probes can hybridize to the same target with efficiencies that vary by several orders of magnitude. The ability to enzymatically generate structure-free single-stranded copies of any nucleic acid without impai...
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acid; CTP32, ATP32, UTP32, and GTP32 for the triphosphates of cytidine, adenosine, uridine, and guanosine labeled with p32 in the ester phosphate; Cp, Ap, Up, and Gp for the 2',3'-nucleoside monophosphates of the bases listed above; PP for inorganic pyrophosphate; Tris, tris-(hydroxymethyl)-aminomethane; TCA, trichloroacetic acid; cpm, counts per minute. 2 Weiss, S. B., and L. Gladstone, J. Am....
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A straightforward enzymatic protocol for converting regular DNA into pseudo-complementary DNA could improve the performance of oligonucleotide microarrays by generating readily hybridizable structure-free targets. Here we screened several highly destabilizing analogs of G and C for one that could be used with 2-aminoadenine (nA) and 2-thiothymine (sT) to generate structure-free DNA that is full...
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Thymidine analogues 5-trifluoromethyl-, 5-pentafluoroethyl- and 5-(heptafluoro-n-propyl)-2'-deoxyuridines were synthesised and converted into the corresponding 5'-triphosphates 1a-c. Performing DNA polymerase-catalyzed primer extension reactions these modified nucleotides were incorporated into DNA to create perfluoroalkylated nucleic acids. Although single modified nucleotides were enzymatical...
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This paper reviews very briefly the recent work on the “structure” of the sodium salt of desoxyribonucleic acid (DNA). By structure is meant not the chemical formula but the spatial arrangement of the atoms. The structure revealed by X-rays is the general structure-that is, the featmes common to DNA from different sources, since the X-rays only respond clearly to the repeating part of the struc...
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ژورنال
عنوان ژورنال: Nucleic Acids Research
سال: 2008
ISSN: 1362-4962,0305-1048
DOI: 10.1093/nar/gkn209